Comparison of Biochemical, Molecular, and Visual Methods to Quantify Phaeocryptopus gaeumannii in Douglas-Fir Foliage
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چکیده
منابع مشابه
Comparison of Biochemical, Molecular, and Visual Methods to Quantify Phaeocryptopus gaeumannii in Douglas-Fir Foliage.
ABSTRACT A recent epidemic of Swiss needle cast along the Oregon coast has prompted efforts to quantify foliar infection and colonization of the causal agent Phaeocryptopus gaeumannii. In this paper, we compare four methods to quantify colonization of Douglas-fir foliage by P. gaeumannii: fruiting body abundance, ergosterol content, dot blot analysis, and TaqMan based real-time quantitative pol...
متن کاملThe histopathology of Phaeocryptopus gaeumannii on Douglas-fir needles.
Germinating ascospores of Phaeocryptopus gaeumannii produce suprastomatal appressoria from which penetration pegs enter needles. Initial infection occurs between late May and early Jul and coincides with budbreak and shoot elongation. Colonization within needles is exclusively intercellular and increases continuously during Jul-May. No intracellular hyphae or haustoria were observed, but hyphae...
متن کاملFluorescence microscopy of Douglas fir foliage epiflora.
BERNSTEIN, M. E. H. M. HOWARD. and G. C. CARROLL. 1973. Fluorescence microscopy of Douglas fir foliage epifloia. Can. J. Microbiol. 19: 1129-1130. Douglas fir needles stained with the fluorescent dye, primulin, were observed microscopically using epiillumination with a mercury arc light source. Microorganisms fell into three categories: algae, which exhibited primary fluorescence; actinomycetes...
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Phaeocryptopus gaeumannii, causal agent of the Douglas-fir foliar disease Swiss needle cast, is the only known pathogenic species of the genus. Current classifications place Phaeocryptopus in the Venturiaceae (Pleosporales), typified by the apple-scab pathogen Venturia inaequalis. All core members of this family have hyphomycetous anamorphs. We sought to confirm these relationships by means of ...
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ژورنال
عنوان ژورنال: Phytopathology®
سال: 2003
ISSN: 0031-949X,1943-7684
DOI: 10.1094/phyto.2003.93.1.121